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Preparation of stock solutions

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Phosphate-buffered saline (PBS)

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Overview
  • Phosphate-buffered saline (PBS) is one of the most suitable biological buffers, extensively used in cell biology and biochemistry laboratory.

  • PBS is a water-based physiological balanced salt solution, which is isotonic and non-toxic to the cells.

  • All the physiological balanced salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885).

  • The first balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution.

  • One of the earliest formulations of phosphate-buffer based salt solution was developed by Dulbacco, and is called Dulbacco's phosphate-buffered saline (DPBS).

  • Since then, the recipe of phosphate-buffered saline was modified and now several formulations exist and are in use.

  • Most of the PBS formulations contain sodium chloride, potassium chloride, sodium phosphate dibasic and potassium phosphate monobasic. Some formulations do not contain potassium chloride.

  • Though several formulations exist, they have physiological range of osmolarity (280-286 mOsm/kg) and pH (7.2 - 7.6).

  • Sodium chloride (and potassium chloride) is added to maintain the osmolarity of the PBS.

  • Sodium phosphate dibasic and potassium phosphate monobasic constitute phosphate buffer system. Sodium phosphate monobasic and sodium phosphate dibasic can also be used as phosphate buffer system. PBS without potassium chloride often use sodium phosphate monobasic and dibasic as buffer system.

  • Phosphate buffer system is one of the buffer systems (others are protein buffer system and bicarbonate buffer system), operated in our body fluid.

  • Depending on the applications, many supplements can be added in the PBS. Most comman supplements are tween-20, EDTA, calcium, magnesium, BSA, fetal bovine serum (FBS), phenol red, glucose, and pyruvate.

Common formulations of PBS
Gel Electrophoresis - Advanced Techniques. Edited by Sameh Magdeldin Gel Electrophoresis - Principles and Basics. Edited by Sameh Magdeldin Genetic Engineering. Edited by Idah Sithole-Niang Genetic Engineering - Basics, New Applications and Responsibilities. Edited by Hugo A. Barrera-Saldaņa Analytical Biochemistry, Third Edition, David Holme and Hazel Peck Applied Biological Engineering - Principles and Practice. Edited by Ganesh R. Naik
See also
Passaging (subculturing) of cells culture | Passaging (subculturing) of adherent cell culture | Passaging (subculturing) of adherent cells culture using Trypsin-EDTA | Bacterial contamination in cell culture | Preparation of Trypsin - EDTA solution | Protocol - Detecting contamination in culture medium | Preparation of Metaphase Chromosome Spreads from adherent cell culture |

Preparation of stock solutions | Preparation of 1M Calcium chloride (CaCl2) solution | Preparation of 1M MOPS solution | Preparation of 0.5M Rubidium chloride (RbCl) solution | Preparation of 1M MOPS solution | Phosphate buffered saline | Preparation of 10X Phosphate buffer saline (PBS) | Preparation of 10X Dulbecco's Phosphate buffered saline (PBS) containing calcium chloride and magnesium chloride |

Preparation of 50X TAE electrophoresis buffer | Agarose gel electrophoresis of DNA | TBE | Tris-EDTA | TAE Vs TBE buffer | Agarose gel electrophoresis of DNA | Preparation of agarose gel for DNA analysis | Agarose gel electrophoresis of DNA | TAE Vs TBE buffer | Agarose gel percentage : DNA fragment size

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