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MOPS stock solution

CaCl2 stock solution

MgCl2 stock solution

MgSO4 stock solution

MnCl2 stock solution

RbCl stock solution

50X TAE electrophoresis buffer

Solution I for plasmid isolation

Solution II for plasmid isolation

Protocol - Plasmid isolation by Boiling lysis

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Agarose gel electrophoresis : Overview

Agarose : Structure

Agarose : Properties

Agarose : Applications

Agarose gel electrophoresis : Electroendosmosis (EEO)

EDTA

Metal chelators

Cell culture antibiotics

Phosphate buffered saline

Plasmid isolation by Alkaline lysis method

Agarose gel electrophoresis of DNA

TAE Vs TBE buffer

Agarose gel percentage : DNA fragment size

DNA fragment size (Base pair) Percentage agarose gel Remarks
0.10 - 1.0 kb 2.0 % To resolve DNA fragments, between 50 - 500 base pair, use polyacrylamide gel
0.2 - 5.0 kb 1.5 %
0.4 - 10.0 kb 1.0 %
0.5 - 12.0 kb 0.8 % 0.8 % - 1.0% agarose gel is very commonly used in our most DNA cloning work.
1.0 - 30.0 kb 0.5 % 0.5% agarose gel is very commonly used for checking genomic DNA purposes. Use pulse field gel electrophoresis to resolve the DNA fragments larger than 30 kb.
  • Preparation of 1M Calcium chloride (CaCl2) solution | Preparation of 50X TAE electrophoresis buffer | Isolation of plasmid by Boiling lysis method: Miniprep | Preparation of 0.5M Rubidium chloride (RbCl) solution | Preparation of 1M MOPS solution | Preparation of Lysis solution (solution II) for the isolation of plasmid | Preparation of resuspension buffer (solution I) for the isolation of plasmid | Plasmid isolation by Alkaline lysis method | Agarose gel electrophoresis of DNA | TAE Vs TBE buffer
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