Agarose gel percentage : DNA fragment size

DNA fragment size (Base pair)	Percentage agarose gel	Remarks
0.10 - 1.0 kb	2.0 %	To resolve DNA fragments, between 50 - 500 base pair, use polyacrylamide gel
0.2 - 5.0 kb	1.5 %
0.4 - 10.0 kb	1.0 %
0.5 - 12.0 kb	0.8 %	0.8 % - 1.0% agarose gel is very commonly used in our most DNA cloning work.
1.0 - 30.0 kb	0.5 %	0.5% agarose gel is very commonly used for checking genomic DNA purposes. Use pulse field gel electrophoresis to resolve the DNA fragments larger than 30 kb.

Updated on 1 January 2013

Related Notes

Protocols and recipes

Preparation of 1M Calcium chloride (CaCl2) solution Preparation of 50X TAE electrophoresis buffer Isolation of plasmid by Boiling lysis method: Miniprep Preparation of 0.5M Rubidium chloride (RbCl) solution

Preparation of 1M MOPS solution Preparation of Lysis solution (solution II) for the isolation of plasmid Preparation of resuspension buffer (solution I) for the isolation of plasmid

BioScience Notes

Plasmid isolation by Alkaline lysis method Agarose gel electrophoresis of DNA TAE Vs TBE buffer .

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